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Novus Biologicals zikv capsid specific antibody

Infection of all three cell types was confirmed by IF detection of the <t>ZIKV</t> C protein (green) at 48 hpi. Infected cells were identified through co-staining with cell-type specific markers (red). While all cultures demonstrated susceptibility to ZIKV infection, (A) neurons exhibited the most pronounced morphological alteration compared to uninfected controls. Notably, viral antigen is localized not only to perinuclear region but also to the axon hillock (arrow) and neurites (arrowhead). In contrast, (B) astrocytes and (C) MBECs maintained their typical cellular architecture despite infection. The highlighted square denotes a region of interest shown at higher magnification. Nuclei were counterstained with DAPI (blue). Scale bars represent 20 µm for neurons and MBECs, and 50 µm for astrocytes. Images are representative of two independent experiments, each comprising three replicates and eight fields analyzed per slide.

Zikv Capsid Specific Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 2 article reviews

zikv capsid specific antibody - by Bioz Stars, 2026-05

93/100 stars

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1) Product Images from "Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo"

Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo

Journal: PLOS One

doi: 10.1371/journal.pone.0337609

Infection of all three cell types was confirmed by IF detection of the ZIKV C protein (green) at 48 hpi. Infected cells were identified through co-staining with cell-type specific markers (red). While all cultures demonstrated susceptibility to ZIKV infection, (A) neurons exhibited the most pronounced morphological alteration compared to uninfected controls. Notably, viral antigen is localized not only to perinuclear region but also to the axon hillock (arrow) and neurites (arrowhead). In contrast, (B) astrocytes and (C) MBECs maintained their typical cellular architecture despite infection. The highlighted square denotes a region of interest shown at higher magnification. Nuclei were counterstained with DAPI (blue). Scale bars represent 20 µm for neurons and MBECs, and 50 µm for astrocytes. Images are representative of two independent experiments, each comprising three replicates and eight fields analyzed per slide.

Figure Legend Snippet: Infection of all three cell types was confirmed by IF detection of the ZIKV C protein (green) at 48 hpi. Infected cells were identified through co-staining with cell-type specific markers (red). While all cultures demonstrated susceptibility to ZIKV infection, (A) neurons exhibited the most pronounced morphological alteration compared to uninfected controls. Notably, viral antigen is localized not only to perinuclear region but also to the axon hillock (arrow) and neurites (arrowhead). In contrast, (B) astrocytes and (C) MBECs maintained their typical cellular architecture despite infection. The highlighted square denotes a region of interest shown at higher magnification. Nuclei were counterstained with DAPI (blue). Scale bars represent 20 µm for neurons and MBECs, and 50 µm for astrocytes. Images are representative of two independent experiments, each comprising three replicates and eight fields analyzed per slide.

Techniques Used: Infection, Staining

Viral RNAs were extracted from both (A) lysates (reflecting intracellular viral load) and (B) culture supernatants (representing extracellular free virus) of primary neurons, astrocytes and MBECs at indicated times post-infection. Absolute RT-qPCR was performed to quantify ZIKV transcripts, with results expressed as “ZIKV copies/mL”. Across all cell types and time points, intracellular viral copy numbers were consistently higher than those detected in the supernatant. Among the three cell types, neurons exhibited the highest levels of both intracellular transcript production and extracellular viral release. Data represents the mean of three independent experiments each performed in duplicate. Statistical analysis was performed using one-way ANOVA followed by Dunnet’s multiple comparison test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Figure Legend Snippet: Viral RNAs were extracted from both (A) lysates (reflecting intracellular viral load) and (B) culture supernatants (representing extracellular free virus) of primary neurons, astrocytes and MBECs at indicated times post-infection. Absolute RT-qPCR was performed to quantify ZIKV transcripts, with results expressed as “ZIKV copies/mL”. Across all cell types and time points, intracellular viral copy numbers were consistently higher than those detected in the supernatant. Among the three cell types, neurons exhibited the highest levels of both intracellular transcript production and extracellular viral release. Data represents the mean of three independent experiments each performed in duplicate. Statistical analysis was performed using one-way ANOVA followed by Dunnet’s multiple comparison test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Techniques Used: Virus, Infection, Quantitative RT-PCR, Comparison

The smaller insets depict light scattering intensity profiles obtained from DLS measurements, while the larger panels present NTA data, illustrating particle concentration peaks (particles/mL) as a function of particle size (diameter) for each analyzed sample from (A) neurons, (B) astrocytes and (C) MBECs. These analyzes demonstrated that ZIKV infection altered both the size distribution and abundance of EVs produced in each culture compared to their respective NIC. Representative data from two independent experiments.

Figure Legend Snippet: The smaller insets depict light scattering intensity profiles obtained from DLS measurements, while the larger panels present NTA data, illustrating particle concentration peaks (particles/mL) as a function of particle size (diameter) for each analyzed sample from (A) neurons, (B) astrocytes and (C) MBECs. These analyzes demonstrated that ZIKV infection altered both the size distribution and abundance of EVs produced in each culture compared to their respective NIC. Representative data from two independent experiments.

Techniques Used: Concentration Assay, Infection, Produced

For each cell type, the upper panel displays a coverage map of ZIKV C11541 genomic regions detected in EVs-GlyR via reference-based assembly of small RNA-seq data. The lower panel illustrates the full-length viral genome (10.8 kb), with blue, purple or green segments indicating regions flanked by sequenced reads. Metrics include percentage genome coverage (CV) and normalized viral nucleotide counts per million total nucleotides (NCPM). No ZIKV sequences were detected in EVs-NIC. To calculate NCPM, we considered as total the number of nucleotides remaining after excluding low-quality and host reads (Neurons: 16’553,501; Astrocytes: 498’011,206; MBECs: 45’213,763). Viral nucleotides were identified as those within this filtered dataset that aligned with the ZIKV C11541 genome (Neurons: 3,907; Astrocytes: 754; MBECs: 9,196). Scale bar: 1 kb. Representative data from three independent experiments.

Figure Legend Snippet: For each cell type, the upper panel displays a coverage map of ZIKV C11541 genomic regions detected in EVs-GlyR via reference-based assembly of small RNA-seq data. The lower panel illustrates the full-length viral genome (10.8 kb), with blue, purple or green segments indicating regions flanked by sequenced reads. Metrics include percentage genome coverage (CV) and normalized viral nucleotide counts per million total nucleotides (NCPM). No ZIKV sequences were detected in EVs-NIC. To calculate NCPM, we considered as total the number of nucleotides remaining after excluding low-quality and host reads (Neurons: 16’553,501; Astrocytes: 498’011,206; MBECs: 45’213,763). Viral nucleotides were identified as those within this filtered dataset that aligned with the ZIKV C11541 genome (Neurons: 3,907; Astrocytes: 754; MBECs: 9,196). Scale bar: 1 kb. Representative data from three independent experiments.

Techniques Used: RNA Sequencing

Immunoperoxidase staining for ZIKV E protein in A549 cell inoculated for 72 h with EVs or ZIKV. (A) Controls included non-infected cells (NIC), mock-treated cells (supernatants from uninfected C6/36HT cells), and supernatants of ZIKV-infected cells at a MOI of 0.1. (B) Brown peroxidase signal indicated productive infection in cells exposed to EVs-IC, while no detectable staining was observed in EVs-GlyR-treated cells. Representative images from three independent experiments, each performed in triplicate. Scale bars: 100 µm for controls and EVs-GlyR) and 50 µm for EVs-IC.

Figure Legend Snippet: Immunoperoxidase staining for ZIKV E protein in A549 cell inoculated for 72 h with EVs or ZIKV. (A) Controls included non-infected cells (NIC), mock-treated cells (supernatants from uninfected C6/36HT cells), and supernatants of ZIKV-infected cells at a MOI of 0.1. (B) Brown peroxidase signal indicated productive infection in cells exposed to EVs-IC, while no detectable staining was observed in EVs-GlyR-treated cells. Representative images from three independent experiments, each performed in triplicate. Scale bars: 100 µm for controls and EVs-GlyR) and 50 µm for EVs-IC.

Techniques Used: Immunoperoxidase Staining, Infection, Staining

A549 monolayers were treated with EVs-Gly or EVs-GlyR. (A) Absolute quantification by RT-qPCR revealed detectable viral RNA in cells treated with EVs-Gly. Statistical analysis was performed using a Mann-Whitney test. (B) Only cells treated with EVs-GlyR derived from neurons or MBECs contained measurable ZIKV RNA, whereas no viral RNA were detected in cells treated with EVs-Gly or GlyR from astrocytes. Statistical analysis was performed using a one-way ANOVA followed by Dunnett’s multiple comparisons test as the post hoc test. (C) ddPCR quantified ZIKV RNA copies directly within EVs preparations (EVs-IC, EVs-Gly, and EVs-GlyR) isolated from infected neurons and MBECs. Dotted lines indicate the ZIKV-positive control (virus diluted 1:10,000, red) and EVs from non-infected control (EVs-NIC, Orange). Data represents the mean of two independent experiments performed in duplicate. Statistical significance: ****p < 0.0001.

Figure Legend Snippet: A549 monolayers were treated with EVs-Gly or EVs-GlyR. (A) Absolute quantification by RT-qPCR revealed detectable viral RNA in cells treated with EVs-Gly. Statistical analysis was performed using a Mann-Whitney test. (B) Only cells treated with EVs-GlyR derived from neurons or MBECs contained measurable ZIKV RNA, whereas no viral RNA were detected in cells treated with EVs-Gly or GlyR from astrocytes. Statistical analysis was performed using a one-way ANOVA followed by Dunnett’s multiple comparisons test as the post hoc test. (C) ddPCR quantified ZIKV RNA copies directly within EVs preparations (EVs-IC, EVs-Gly, and EVs-GlyR) isolated from infected neurons and MBECs. Dotted lines indicate the ZIKV-positive control (virus diluted 1:10,000, red) and EVs from non-infected control (EVs-NIC, Orange). Data represents the mean of two independent experiments performed in duplicate. Statistical significance: ****p < 0.0001.

Techniques Used: Quantitative Proteomics, Quantitative RT-PCR, MANN-WHITNEY, Derivative Assay, Isolation, Infection, Positive Control, Virus, Control

Brains from EVs-inoculated mice were analyzed to assess ZIKV infection. (A) Western blot analysis of whole brain lysates confirmed ZIKV C protein (~12 kDa) in mice inoculated with EVs- GlyR derived from neurons (EVs-N) or MBECs (EVs-EC), as well as in ZIKV-infected positive controls. C protein signal was absent in mock-treated, or EVs-NIC–inoculated mice. β-actin (~42 KDa) served as loading control. (B) ZIKV RNA copies quantified by RT-qPCR in whole-brain lysates revealed significantly higher viral RNA loads in mice receiving neuron-derived EVs-GlyR compared to MBECs-derived EVs-GlyR. Representative data from three independent experiments performed in duplicate. Statistical analysis was performed using a Mann-Whitney test (p < 0.05 threshold), but no significant differences were detected.

Figure Legend Snippet: Brains from EVs-inoculated mice were analyzed to assess ZIKV infection. (A) Western blot analysis of whole brain lysates confirmed ZIKV C protein (~12 kDa) in mice inoculated with EVs- GlyR derived from neurons (EVs-N) or MBECs (EVs-EC), as well as in ZIKV-infected positive controls. C protein signal was absent in mock-treated, or EVs-NIC–inoculated mice. β-actin (~42 KDa) served as loading control. (B) ZIKV RNA copies quantified by RT-qPCR in whole-brain lysates revealed significantly higher viral RNA loads in mice receiving neuron-derived EVs-GlyR compared to MBECs-derived EVs-GlyR. Representative data from three independent experiments performed in duplicate. Statistical analysis was performed using a Mann-Whitney test (p < 0.05 threshold), but no significant differences were detected.

Techniques Used: Infection, Western Blot, Derivative Assay, Control, Quantitative RT-PCR, MANN-WHITNEY

IF analysis localized ZIKV C protein (red) in MAP2-positive neurons and GFAP-positive astrocytes in brains of EVs-GlyR-inoculated mice. Activated astrocytes (arrowheads) and ZIKV antigen in capillary-associated endothelial cells (arrows) were observed across all treatment groups. Scale bar: 20 µm. Images are representative of four inoculated animals per group.

Figure Legend Snippet: IF analysis localized ZIKV C protein (red) in MAP2-positive neurons and GFAP-positive astrocytes in brains of EVs-GlyR-inoculated mice. Activated astrocytes (arrowheads) and ZIKV antigen in capillary-associated endothelial cells (arrows) were observed across all treatment groups. Scale bar: 20 µm. Images are representative of four inoculated animals per group.

Techniques Used:

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Journal: PLOS One

Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo

doi: 10.1371/journal.pone.0337609

Figure Lengend Snippet: Infection of all three cell types was confirmed by IF detection of the ZIKV C protein (green) at 48 hpi. Infected cells were identified through co-staining with cell-type specific markers (red). While all cultures demonstrated susceptibility to ZIKV infection, (A) neurons exhibited the most pronounced morphological alteration compared to uninfected controls. Notably, viral antigen is localized not only to perinuclear region but also to the axon hillock (arrow) and neurites (arrowhead). In contrast, (B) astrocytes and (C) MBECs maintained their typical cellular architecture despite infection. The highlighted square denotes a region of interest shown at higher magnification. Nuclei were counterstained with DAPI (blue). Scale bars represent 20 µm for neurons and MBECs, and 50 µm for astrocytes. Images are representative of two independent experiments, each comprising three replicates and eight fields analyzed per slide.

Article Snippet: Following inoculum removal, cells were maintained in fresh medium for 48 h. Infection was confirmed via IF assay using the ZIKV capsid-specific antibody (Novus, NBP3–13200) as described before, and absolute RT-qPCR.

Techniques: Infection, Staining

Journal: PLOS One

Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo

doi: 10.1371/journal.pone.0337609

Figure Lengend Snippet: Viral RNAs were extracted from both (A) lysates (reflecting intracellular viral load) and (B) culture supernatants (representing extracellular free virus) of primary neurons, astrocytes and MBECs at indicated times post-infection. Absolute RT-qPCR was performed to quantify ZIKV transcripts, with results expressed as “ZIKV copies/mL”. Across all cell types and time points, intracellular viral copy numbers were consistently higher than those detected in the supernatant. Among the three cell types, neurons exhibited the highest levels of both intracellular transcript production and extracellular viral release. Data represents the mean of three independent experiments each performed in duplicate. Statistical analysis was performed using one-way ANOVA followed by Dunnet’s multiple comparison test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

Techniques: Virus, Infection, Quantitative RT-PCR, Comparison

Journal: PLOS One

Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo

doi: 10.1371/journal.pone.0337609

Figure Lengend Snippet: The smaller insets depict light scattering intensity profiles obtained from DLS measurements, while the larger panels present NTA data, illustrating particle concentration peaks (particles/mL) as a function of particle size (diameter) for each analyzed sample from (A) neurons, (B) astrocytes and (C) MBECs. These analyzes demonstrated that ZIKV infection altered both the size distribution and abundance of EVs produced in each culture compared to their respective NIC. Representative data from two independent experiments.

Techniques: Concentration Assay, Infection, Produced

Journal: PLOS One

Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo

doi: 10.1371/journal.pone.0337609

Figure Lengend Snippet: For each cell type, the upper panel displays a coverage map of ZIKV C11541 genomic regions detected in EVs-GlyR via reference-based assembly of small RNA-seq data. The lower panel illustrates the full-length viral genome (10.8 kb), with blue, purple or green segments indicating regions flanked by sequenced reads. Metrics include percentage genome coverage (CV) and normalized viral nucleotide counts per million total nucleotides (NCPM). No ZIKV sequences were detected in EVs-NIC. To calculate NCPM, we considered as total the number of nucleotides remaining after excluding low-quality and host reads (Neurons: 16’553,501; Astrocytes: 498’011,206; MBECs: 45’213,763). Viral nucleotides were identified as those within this filtered dataset that aligned with the ZIKV C11541 genome (Neurons: 3,907; Astrocytes: 754; MBECs: 9,196). Scale bar: 1 kb. Representative data from three independent experiments.

Techniques: RNA Sequencing

Journal: PLOS One

Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo

doi: 10.1371/journal.pone.0337609

Figure Lengend Snippet: Immunoperoxidase staining for ZIKV E protein in A549 cell inoculated for 72 h with EVs or ZIKV. (A) Controls included non-infected cells (NIC), mock-treated cells (supernatants from uninfected C6/36HT cells), and supernatants of ZIKV-infected cells at a MOI of 0.1. (B) Brown peroxidase signal indicated productive infection in cells exposed to EVs-IC, while no detectable staining was observed in EVs-GlyR-treated cells. Representative images from three independent experiments, each performed in triplicate. Scale bars: 100 µm for controls and EVs-GlyR) and 50 µm for EVs-IC.

Techniques: Immunoperoxidase Staining, Infection, Staining

Journal: PLOS One

Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo

doi: 10.1371/journal.pone.0337609

Figure Lengend Snippet: A549 monolayers were treated with EVs-Gly or EVs-GlyR. (A) Absolute quantification by RT-qPCR revealed detectable viral RNA in cells treated with EVs-Gly. Statistical analysis was performed using a Mann-Whitney test. (B) Only cells treated with EVs-GlyR derived from neurons or MBECs contained measurable ZIKV RNA, whereas no viral RNA were detected in cells treated with EVs-Gly or GlyR from astrocytes. Statistical analysis was performed using a one-way ANOVA followed by Dunnett’s multiple comparisons test as the post hoc test. (C) ddPCR quantified ZIKV RNA copies directly within EVs preparations (EVs-IC, EVs-Gly, and EVs-GlyR) isolated from infected neurons and MBECs. Dotted lines indicate the ZIKV-positive control (virus diluted 1:10,000, red) and EVs from non-infected control (EVs-NIC, Orange). Data represents the mean of two independent experiments performed in duplicate. Statistical significance: ****p < 0.0001.

Techniques: Quantitative Proteomics, Quantitative RT-PCR, MANN-WHITNEY, Derivative Assay, Isolation, Infection, Positive Control, Virus, Control

Journal: PLOS One

Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo

doi: 10.1371/journal.pone.0337609

Figure Lengend Snippet: Brains from EVs-inoculated mice were analyzed to assess ZIKV infection. (A) Western blot analysis of whole brain lysates confirmed ZIKV C protein (~12 kDa) in mice inoculated with EVs- GlyR derived from neurons (EVs-N) or MBECs (EVs-EC), as well as in ZIKV-infected positive controls. C protein signal was absent in mock-treated, or EVs-NIC–inoculated mice. β-actin (~42 KDa) served as loading control. (B) ZIKV RNA copies quantified by RT-qPCR in whole-brain lysates revealed significantly higher viral RNA loads in mice receiving neuron-derived EVs-GlyR compared to MBECs-derived EVs-GlyR. Representative data from three independent experiments performed in duplicate. Statistical analysis was performed using a Mann-Whitney test (p < 0.05 threshold), but no significant differences were detected.

Techniques: Infection, Western Blot, Derivative Assay, Control, Quantitative RT-PCR, MANN-WHITNEY

Journal: PLOS One

Article Title: Neural and endothelial cell-derived extracellular vesicles mediate Zika virus genome dissemination and productive infection in vivo

doi: 10.1371/journal.pone.0337609

Figure Lengend Snippet: IF analysis localized ZIKV C protein (red) in MAP2-positive neurons and GFAP-positive astrocytes in brains of EVs-GlyR-inoculated mice. Activated astrocytes (arrowheads) and ZIKV antigen in capillary-associated endothelial cells (arrows) were observed across all treatment groups. Scale bar: 20 µm. Images are representative of four inoculated animals per group.

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